FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367316
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17420.12740288272791367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41200.037.1774181
GGTATCA18650.033.774481
GTGGTAT7600.032.155941
TATCAAC47400.032.0743682
ATCAACG48350.031.6286223
TCAACGC48300.031.5786614
AACGCAG48750.031.2894526
CAACGCA49050.031.028045
ACGCAGA52550.029.0922227
CGCAGAG54550.028.0255978
AGAGTAC53750.027.87257411
CAGAGTA54500.027.54802510
TGGTATC8950.027.2866022
GCAGAGT58850.026.0362269
AGTACGG46200.024.10279813
GAGTACG46750.023.88750512
TAACCGA750.002042634422.9033165
AGTACTT18100.021.26688213
GTACGGG52900.020.59529514
GAGTACT18700.020.39625512
Produced by FastQC (version 0.11.5)