FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847156
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG200.00700967952.3892458
GTATCAA14350.042.3658681
GGTATCA6450.039.0947531
ATCAACG18300.033.4021953
TCAACGC18250.033.1152464
CAACGCA19100.031.64345
TATCAAC19250.031.2155722
AACGCAG19850.030.4478036
ACGCAGA21650.028.0742117
CGTCATA500.007687328427.6341021
TGGTATC2500.027.6275772
AGAGTAC21600.027.4996711
GTGGTAT3150.026.3181921
CAGAGTA22950.026.0325210
CGCAGAG23350.025.8823648
GCAGAGT24800.024.3690839
GAGTACT9800.022.90416312
CGAACCT2004.5474735E-1122.64792653
CCGCTAT1602.5561175E-422.37924870
AGTACTT10400.021.58277113
Produced by FastQC (version 0.11.5)