FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847156
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG250.0090202649.1505369
GTATCAA16050.037.650491
GGTATCA8250.033.065931
ATCAACG18300.032.774483
TCAACGC18400.032.5828864
CAACGCA18650.032.1461145
TATCAAC18900.031.9465792
AACGCAG19400.031.2585686
GTGGTAT3650.030.2736471
ACGCAGA21450.028.5924157
AGAGTAC22150.026.60464111
CAGAGTA22700.026.10876710
CGCAGAG23400.026.0624688
GCAGAGT24100.025.4484359
TGGTATC4250.025.1663882
TAGCGGC2250.024.99742164
AATAGCG2250.024.9403562
AGTACGG18150.023.17245713
GAGTACG18700.022.30261212
CAGTTCG1400.002690104821.83953370
Produced by FastQC (version 0.11.5)