FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797629
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.042.5143781
TCAACGC18150.036.0179024
ATCAACG18450.035.4322473
CAACGCA18700.034.9585535
TATCAAC18650.034.681352
GGTATCA6250.034.322671
AACGCAG19300.033.8696376
ACGCAGA20250.031.9390957
TTACGAT450.004565611530.7435156
AGAGTAC20600.030.21927611
CGCAGAG21250.030.1105588
CCTATAC1501.8189894E-1229.9768053
CAGAGTA21400.029.08958610
GCAGAGT23250.027.6674279
AGTACGG17700.024.22853713
GTATAAG3150.024.1647071
GAGTACG17850.024.02493712
TGGTATC3050.023.8151152
GAGTACT8200.023.61853612
TACGGGT2850.023.05618915
Produced by FastQC (version 0.11.5)