FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154170
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG300.00392355460.661570
GTATCAA27800.039.6923261
CTCGTAT1250.038.76678539
GGTATCA11850.036.665161
TCGTATG1400.034.63278240
ATCAACG33450.033.1869283
TCAACGC33900.032.746394
CAACGCA34400.032.270435
TATCAAC35100.031.7250792
AACGCAG35600.031.1826636
GTGGTAT5250.029.5566081
CGTATGC1650.029.41099241
TGGTATC5400.028.7293682
ACGCAGA38650.028.720697
CGCAGAG39500.028.102658
AGAGTAC39000.027.93257311
TATGTCG500.007758795727.58258220
CAGAGTA39700.027.52689710
GCAGAGT43900.025.4430399
GAGTACG34600.022.81643912
Produced by FastQC (version 0.11.5)