FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154170
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15600.1351620645138931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6200.042.7790642
GTGGTAT6600.040.7471771
GGTATCA15050.038.7165871
GTATCAA31950.036.0431251
TATCAAC38450.030.0110172
ATCAACG38550.029.6445373
TCAACGC39100.029.3066844
CAACGCA39400.029.1708765
AACGCAG40750.028.3745986
ACGCAGA43950.026.3086417
CGCAGAG44500.025.9812288
AGAGTAC44150.025.72402411
CAGAGTA45900.025.26373910
CGGAATA850.001458651524.26688269
GCAGAGT48300.024.079649
GAGTACG40050.021.65571612
AGTACGG39750.021.64786113
GTACATG6150.019.6218411
AGTACTT13950.019.24564713
GCTTAGA1651.8393584E-618.8063891
Produced by FastQC (version 0.11.5)