FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161544
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22920.1973235624306957No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22790.19620436246926506No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21880.1883699627392505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21830.18793950121562333No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20110.17313162480284863No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19400.1670190711673428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18920.16288664054052193No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18530.15952904065622997No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGTAGATGCA17700.1523833793640189No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17440.15014497944115765No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTT16330.1405887336166344No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15860.136542395294539No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15810.13611193377091182No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15740.13550928763783376No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15620.13447617998112857No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15120.13017156474485683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15050.1295689186117788No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14600.12569476489913425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14270.12285371884319492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14160.12190670349121514No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13950.12009876509198102No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13680.1177742728643943No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13360.11501931911318039No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13340.11484713450372952No Hit
CTATATTACTGTGCGAAAAACTCCTATGGAGCAGTGGGTCCAAGGGCTTT13170.11338356532339713No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13130.1130391961044954No Hit
GTCCAGGGAAGGGGCTGGAGTGGGTCTCATTTATTAGTGGTAGTGGTGAT12970.11166171922888846No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12830.11045642696273236No Hit
TCCCTGGACCCTGGCGGACCCAACTCATGACATAATTGTTAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCT12510.10770147321151848No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12450.10718491938316585No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12400.10675445785953867No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGTAGATG12230.10529088867920629No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12220.10520479637448085No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12200.10503261176503No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12160.10468824254612825No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11880.10227765801381611No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11880.10227765801381611No Hit
ATATTACTGTGCGAAAAACTCCTATGGAGCAGTGGGTCCAAGGGCTTTTGATGTCTGGGGCCAAGGGACAATGGT11790.10150282727128719No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11770.1013306426618363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG200.007154776752.1197347
GTGGTAT3000.044.7327731
GTATCAA23350.037.136091
CTTATAC1154.9112714E-1132.913751
TATCAAC27000.032.106162
TCAACGC27700.031.5431864
ATCAACG27600.031.4082013
CAACGCA28000.031.2065665
CGCTAAT1001.4711986E-831.00355532
AACGCAG28500.030.7797876
TCCAACG450.004663559630.61155931
CGCAGAG30850.028.5454188
AGAGTAC31050.028.02918811
CAGAGTA31450.027.67269710
ACGAGAC1155.7842044E-826.9294322
GGTATCA17350.026.575891
GCAGAGT33300.026.2386269
ACGGGAC951.0751948E-525.34587916
ACGCAGA34750.025.242777
TATACAC1501.0786607E-925.2262693
Produced by FastQC (version 0.11.5)