FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026782
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43650.040.427321
GGTATCA18850.039.31121
TATCAAC51450.033.8202932
TCAACGC51750.033.6225744
ATCAACG52550.033.2410283
CAACGCA52600.033.0137375
AACGCAG54600.031.8044456
ATCGTAC553.1439884E-431.55454845
AGAGTAC56600.029.58414611
ACGCAGA60800.028.5038577
CGCAGAG62450.027.8059228
CAGAGTA60550.027.71180210
GCAGAGT68300.025.6260819
AGTACGG55050.024.40880213
GAGTACG55950.024.2624912
CGTATAC750.00200908222.9693093
GTGGTAT6800.022.8082961
CGTAATA953.0899022E-421.76523223
TTAACGG1605.6525096E-821.58805335
GTACGGG62800.021.23198114
Produced by FastQC (version 0.11.5)