FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409798
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59500.038.2493971
GGTATCA26750.037.38281
ATCAACG69400.032.9346123
TCAACGC70150.032.5333484
TATCAAC70700.032.23152
CAACGCA71500.031.9679625
AACGCAG73600.031.0558346
GTGGTAT12000.029.3100471
TAGACCG953.2264688E-729.0317945
ACGCAGA80150.028.558547
CGCAGAG83300.027.4785968
AGAGTAC82900.027.40326511
CAGAGTA84550.026.82771710
GCAGAGT89700.025.5564569
TGGTATC14000.024.8709182
AGTACGG70450.024.17176613
GAGTACG71600.023.68724312
TTAACGT800.00291279521.5465874
GTACGGG83750.020.04502314
TCTAACG900.00540999119.36184353
Produced by FastQC (version 0.11.5)