FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651565
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.038.819271
GGTATCA7800.036.949191
ATCAACG20550.033.0577743
GTGGTAT2300.032.8182221
TCAACGC20750.032.7391474
CAACGCA21000.032.5127755
AACGCAG21300.031.7326896
TATCAAC21550.031.5237712
ACGCAGA22750.029.7056067
AGAGTAC22600.029.29560311
CAGAGTA23050.028.72367110
CGCAGAG23650.028.720218
GTAACGC500.007498725327.77412242
GCAGAGT24700.027.499319
TCATTAC756.395578E-527.447973
ATGTGTA1503.6379788E-1127.44375811
ATAATAC658.936666E-426.3922793
TGGTATC2800.025.7324712
CGTCAGA1359.980795E-925.4147855
AGTACGG21050.025.09702313
Produced by FastQC (version 0.11.5)