FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242725
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53770.432678187048623No Hit
CTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA47750.384236255004124No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC37830.3044116759540526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34140.27471886378724175No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33030.26578687963950187No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC32310.2599931601923193No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29960.2410831036633205No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28950.2329558027721338No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGGTTCATGGAGA28540.22965660142026592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28330.22796676658150436No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC27760.22338007201915147No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26830.21589651773320728No Hit
GTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGAGCTGGATCCG26310.21171216479913096No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26190.21074654489126715No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24730.19899816934559134No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCTGGCACTGATTTCACATTGAA23850.19191695668792372No Hit
GCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23320.1876521354281921No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23090.18580136393811986No Hit
GTCTCCACTCTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA22320.1796053028626607No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT22270.17920296123438412No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21990.17694984811603534No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21590.17373111508982278No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21190.17051238206361022No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC21050.16938582550443582No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG20830.16761552234001892No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA20340.16367257438290853No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGA20210.16262648614938946No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20180.1623850811724235No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19350.15570621014303246No Hit
GTCTGGCACTGATTTCACATTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAG19130.15393590697861556No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19040.15321169204771773No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT18970.15264841376813051No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18660.15015389567281578No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18630.14991249069584983No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18170.1462109477157054No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA18150.14605001106439477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18120.14580860608742885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17850.14363596129473535No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17810.1433140879921141No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTG17520.14098050654811No Hit
GTGTATTACTGTGCGAGAGAAAGGCGTTCCTATGATATTAGTGGTTACCC17500.14081956989679936No Hit
CTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCA17490.14073910157114405No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17330.13945160836065904No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17140.13792271017320806No Hit
GTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGT16860.1356695970548593No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16660.134060230541753No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16330.13140477579512763No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16300.1311633708181617No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15570.12528918304532377No Hit
AGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15470.12448449978877064No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15330.12335794322959626No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15060.12118529843690277No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14980.12054155183166025No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14920.12005874187772839No Hit
GGTCTAGTCAAAGCCTCGTACACAATGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGTCTC14890.11981733690076245No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCATTGTGT14750.11869078034158803No Hit
GTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGC14740.11861031201593274No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14690.11820797038765615No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14370.11563298396668611No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14230.11450642740751171No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14170.11402361745357983No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14040.11297752922006077No Hit
CTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA13870.11160956768392041No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13790.1109658210786779No Hit
GTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG13690.11016113782212476No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13570.10919551791426099No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13510.10871270796032911No Hit
CAATGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGT13130.10565491158542718No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12900.10380414009535496No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12850.1034017984670784No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12740.10251664688486993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12710.10227524190790399No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12570.1011486853487296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.042.2388081
GGTATCA9550.041.9226761
GTGGTAT3300.041.835061
ACGCGTA602.8915565E-740.2840827
TACTGAT11250.038.5060170
TGGTATC3600.037.3840682
TATCAAC28500.035.8402752
CTCGTAT709.506548E-734.6058539
AGACGCG709.691139E-734.5208725
ATCAACG30150.034.1077773
CAACGCA30650.033.663965
TCAACGC30650.033.551374
AACGCAG31050.033.341426
AATCTCG751.6428039E-632.26624336
GCGTAGT901.9792606E-730.69510729
CGCAGAG33750.030.6691688
AGAGTAC33700.030.61229111
TCGTATG802.6865437E-630.2947940
ACGGGTA802.7575334E-630.19360716
CAGAGTA34600.029.81601710
Produced by FastQC (version 0.11.5)