FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242725
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51190.411917359029552No Hit
CTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA40670.32726468044016177No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC34550.2780180651391096No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC34480.2774547868595224No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32110.25838379367921305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29690.23891045887062703No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29060.23384095435434227No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGGTTCATGGAGA27800.2237019453217727No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26920.2166207326641051No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26220.21098794986823313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25970.20897624172685025No Hit
GTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGAGCTGGATCCG25770.20736687521374397No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC25390.20430907883884206No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25120.20213643404614856No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24500.19714739785551913No Hit
GTCTCCACTCTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA23470.1888591603130218No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22500.18105373272445632No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22270.17920296123438412No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCTGGCACTGATTTCACATTGAA21940.17654750648775874No Hit
GCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20200.16254601782373412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20190.16246554949807882No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG20090.16166086624152567No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19860.15981009475145347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19610.15779838661007062No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19450.1565108933995856No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA19440.15643042507393026No Hit
GTGTATTACTGTGCGAGAGAAAGGCGTTCCTATGATATTAGTGGTTACCC19040.15321169204771773No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18930.15232654046550925No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18750.15087811060371362No Hit
GTCTGGCACTGATTTCACATTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAG18720.1506367056267477No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC18700.15047576897543705No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT18620.14983202237019452No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18510.14894687078798607No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18350.14765937757750106No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGA18100.1456476694361182No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17780.14307268301514817No Hit
GTGCCAGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGGTT17730.1426703413868716No Hit
CTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCA17270.13896879840672716No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTG16990.13671568528837835No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16990.13671568528837835No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT16800.13518678710092738No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16580.13341648393651048No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16560.13325554728519987No Hit
GTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGT16430.13220945905168077No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16430.13220945905168077No Hit
GTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGC16350.13156571244643825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.12786416946629384No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15650.1259329296505663No Hit
CTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA15540.12504777806835785No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15370.12367981653221752No Hit
AGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15160.1219899816934559No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA15080.1213462350882134No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCATTGTGT15050.12110483011124745No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14890.11981733690076245No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14830.11933452694683055No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.11724235047979238No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14220.1144259590818564No Hit
GGTCTAGTCAAAGCCTCGTACACAATGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGTCTC14190.11418455410489045No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14090.11337987084833732No Hit
GTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG13750.11064394777605664No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13730.11048301112474603No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13690.11016113782212476No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13520.10879317628598442No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13440.10814942968074193No Hit
GGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAGCTACACAC13430.1080689613550866No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13330.10726427809853348No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.10517210163149532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12950.10420648172363153No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12740.10251664688486993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT305.996718E-446.48022556
GTGGTAT3250.044.6050641
GGTATCA10000.043.83511
TGGTATC3450.041.983722
GTATCAA25900.041.9787061
TATCAAC28350.038.4401172
ATCAACG30350.036.106623
CAACGCA30550.035.7444955
TCAACGC30850.035.3983234
AACGCAG31300.034.9980556
CGCAGAG33750.032.7636578
AGAGTAC33850.032.46594611
GCAGAGT34750.031.9186659
CAGAGTA34550.031.90403410
ACGCAGA34650.031.8132367
TACTGAT9800.028.1142170
CGATTGT500.00717767328.02622262
TTTCACG500.007503112327.77278146
GCGATAG500.007666367627.65056236
GTATTAG2000.027.612661
Produced by FastQC (version 0.11.5)