FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820762
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36100.043.1679841
TCAACGC42450.036.5347374
ATCAACG42550.036.36873
GGTATCA14450.035.8687171
CAACGCA43350.035.855915
TATCAAC43600.035.5730482
AACGCAG44750.034.7341656
ACGCAGA49150.031.622097
CGCAGAG51050.030.5804778
AGAGTAC49750.030.5464811
CAGAGTA51250.029.85461210
GTGGTAT6350.029.3841061
GCAGAGT53100.029.3348339
TGGTATC7500.024.8710272
AGTACGG44300.024.8707313
GAGTACG44850.024.64274612
AATAGCG4550.023.83798662
TTAATCG4500.022.30828337
ACGAACC4650.021.70935852
ATAGCGG4700.021.6075863
Produced by FastQC (version 0.11.5)