FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772857
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG300.00434632659.11097370
GTATCAA16650.038.797761
GGTATCA7600.036.81721
CGTACCC400.002582719334.535593
ATCAACG19250.033.3694533
TCAACGC19300.033.2830054
TATCAAC19850.032.5347862
CAACGCA19750.032.524665
CGTCCTA553.2180414E-431.42649533
AACGCAG20550.031.2584886
TATACGA450.00455644730.75601636
ATATACG450.00459851230.6983014
ACGCAGA21050.030.5120587
AGAGTAC21250.030.2229311
CGCAGAG21650.029.666468
CAGAGTA22900.028.34685910
GTACTAG500.0076848527.6356241
GCAGAGT23800.027.274929
TAACGGC1950.026.61578236
TTAACGG2000.025.9419835
Produced by FastQC (version 0.11.5)