FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772857
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17850.040.471451
GGTATCA7850.039.1887171
ATCAACG19300.036.6479343
CAACGCA19900.035.3581435
TCAACGC19950.035.269534
ATAACGC501.8600038E-434.5026863
TATCAAC20550.034.458882
AACGCAG20750.034.4084136
ACGCAGA22150.032.389337
CGCAGAG22350.031.9451718
AGAGTAC22300.030.93810511
CAGAGTA23150.029.79829210
GCAGAGT24000.029.748949
GTCTATA1052.2864697E-829.6274261
AGTACGG18650.026.8234113
GAGTACG18600.026.708312
CCGATCG1001.565777E-524.1440689
TTAACGG1602.1427695E-923.7713735
ATTCCGC1352.6383896E-723.0166928
GTACGGG21600.023.00179114
Produced by FastQC (version 0.11.5)