FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198107
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41180.34370886740499806No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGATTCAGGGAGA35840.2991385577415039No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30130.25148004310132566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29960.2500611381120384No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29110.24296661316560209No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26370.2200972033382661No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA25850.21575702337103447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25660.21417118838300753No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25640.21400425838426787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24750.20657587344035216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23700.197812048506519No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23620.19714432851156033No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22160.18495843860356379No Hit
GAGTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21310.17786391365712745No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20950.17485917367981324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20840.173941058686745No Hit
GTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA20730.17302294369367677No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20490.1710197837088006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAG20330.1696843437188832No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20270.16918355372266417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20160.16826543872959593No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20110.16784811373274675No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20020.16709692873841817No Hit
GTGTATTACTGTGCGAGGGGCGTTCATAGAAGCAGCTGGACGGGGCGTTTCTTAGTGGGCGGTCCCTACTTTGA19610.16367486376425477No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19580.16342446876614528No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18720.15624647882033907No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18620.1554118288266407No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18290.152657483847436No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17900.14940234887201226No Hit
GTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17740.14806690888209484No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17650.1473157238877663No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17420.14539602890226No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGATTCAGGGA16830.14047159393943948No Hit
GTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16520.13788417895897445No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16340.13638180897031735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15970.1332936039936333No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT15930.13295974399615393No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15810.13195816400371588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15610.1302888640163191No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15350.12811877403270325No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15140.12636600904593664No Hit
TTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15130.12628254404656677No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14920.12452977905980016No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14780.12336126906862242No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14710.12277701407303354No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA14630.12210929407807482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14540.12135810908374628No Hit
GTAGTAGGCGAGCCCACTGAAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCT14440.12052345909004789No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTTCGGCCAAGGGA14320.1195218790976098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13610.11359586414235122No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTT13410.11192656415495443No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13400.1118430991555846No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13230.11042419416629733No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13200.11017379916818781No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13150.10975647417133862No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGA13100.10933914917448942No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACC13020.1086714291795307No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12860.10733598918961328No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12780.10666826919465457No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12720.10616747919843554No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12700.10600054919969586No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12660.1056666892022165No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT12640.10549975920347682No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12620.10533282920473713No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12550.10474857420914825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12410.10358006421797052No Hit
GAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG12300.1026619492249023No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12290.10257848422553244No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12280.10249501922616261No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC12270.10241155422679278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)