FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978751
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18340.18738167317325857No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACGGAGTTCAGGATTAGGGAGAACTCATTCTTGGACATGTCTACGGA18200.1859512787215543No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15950.1629627964620215No Hit
GTGTATTACTGTGCGAGATCTCCCCTTACGAAACTTTGGCGGGGCGCCAA14260.14569589200930574No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14170.14477635271892442No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14170.14477635271892442No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13690.1398721431702241No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12550.1282246454920608No Hit
ATCTATTATAGTGGGGTCACCTACTACAGCCCGTCCCTCAAGAGTCGAGT11800.12056181807221653No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11350.11596412162030996No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA10440.10666655768423225No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG10280.10503182116799882No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9810.10022978265156306No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9790.10002544058703387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31000.042.2360531
TCGTATG1200.037.5072440
TATCAAC35350.037.135652
ATCAACG35850.036.8988843
TCAACGC36000.036.7432634
CAACGCA36400.036.1512035
CGTATGC1250.036.03856741
AACGCAG37250.035.418276
GGTATCA16150.033.5325241
CGCAGAG39600.033.3164338
GTGGTAT2900.033.0934221
AGAGTAC40650.032.37155511
TCGACGT1500.032.0607529
GCAGAGT41350.031.823559
CAGAGTA41400.031.78511410
CTCGTAT1207.4578566E-1131.70253239
TACGGCA553.387911E-431.1530234
ACGCTAC450.004305044731.11563552
CGACGTC1550.031.03765930
GAGTACG33150.030.081712
Produced by FastQC (version 0.11.5)