FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978751
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.2010725914967137No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACGGAGTTCAGGATTAGGGAGAACTCATTCTTGGACATGTCTACGGA18690.19095765930251923No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17850.18237529259229365No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16630.16991042665601364No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14790.15111095671932903No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13240.13527444671831754No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11910.12168569942712702No Hit
GTGTATTACTGTGCGAGATCTCCCCTTACGAAACTTTGGCGGGGCGCCAA11870.12127701529806867No Hit
ATCTATTATAGTGGGGTCACCTACTACAGCCCGTCCCTCAAGAGTCGAGT11260.11504458232992866No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11050.11289899065237226No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11020.1125924775555785No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10230.10452096600667585No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9930.10145583503873815No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA9810.10022978265156306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33200.043.630481
GTGGTAT3500.043.0499231
TATCAAC37150.039.047652
ATCAACG38100.037.9609383
TCAACGC38650.037.4188274
CAACGCA39200.036.893825
AACGCAG39650.036.4732366
CGCAGAG40850.035.319928
AGAGTAC41000.035.02392211
CAGAGTA41600.034.59918610
GCAGAGT42100.034.2694789
GGTATCA15700.032.063091
ACGCAGA45800.031.6502747
CGTAGCG450.00429482431.13074552
GAGTACG37500.030.54308112
AGTACGG37650.030.51220713
ACGGGAT5500.028.60551616
GAGTACT10100.028.09674612
GTACGGG40700.027.8056414
TACGGGG17250.027.74983615
Produced by FastQC (version 0.11.5)