FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593776
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82580.518140566804871No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55020.3452178976217486No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52260.3279005330736565No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51960.32601821084016824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50520.31698306411942456No Hit
GCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT49850.31277921113130075No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC49720.31196353816345584No Hit
CTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC48480.30418327293170433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47240.2964030076999528No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42570.2671015249319854No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA42480.2665368282619389No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT42270.26521920269849714No Hit
GATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA41860.2626466956460632No Hit
CTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTT41430.25994870044473No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC41270.25894479525353625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40600.25474094226541244No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39880.2502233689050406No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39850.25003513668169175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39840.24997239260724216No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39710.24915671963939723No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTTATAGTTTT39320.2467097007358625No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38720.24294505626888596No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT38670.2426313358966379No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38310.240372549216452No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37250.23372167732479346No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36590.22958056841111923No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT36280.22763550210318137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36110.22656885283753805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35110.22029444539257712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34720.2178474264890424No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT33940.21295338868197286No Hit
ATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA32670.20498489122687252No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31940.20040457379205107No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC30690.19256156448584996No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTA30600.19199686781580347No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT29750.18666362148758672No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC29730.1865381333386875No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC29050.1822715362761141No Hit
ACTTATTCCTGTCAACAGCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA28200.17693828994789731No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG27570.17298541325757197No Hit
ACCTTGATGGGACCCCATTTTGTAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG27530.17273443695977353No Hit
GGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCA27470.17235797251307586No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC27470.17235797251307586No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27200.1706638825029364No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27130.17022467398178914No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26460.16602082099366536No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG26340.16526789210027004No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26320.16514240395137084No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26160.16413849876017708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25630.16081306281434782No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT24970.15667195390067362No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24760.15535432833723184No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24310.15253084498699943No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23840.1495818734878678No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTGTAAAGT23640.14832699199887564No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23190.14550350864864323No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCA22940.14393490678740298No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22890.14362118641515495No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22780.14293100159620925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22160.1390408689803335No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21590.13546445673670576No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21540.13515073636445774No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21430.13446055154551204No Hit
GCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGG21370.1340840870988144No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21360.13402134302436478No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA21250.13333115820541908No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21240.13326841413096946No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21210.13308018190762066No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20840.13075865115298513No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20770.13031944263183784No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20730.13006846633403943No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCA20680.12975474596179137No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20420.12812340002610154No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20340.12762144743050466No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20250.1270567507604582No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20120.12624107779261326No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19940.1251116844525203No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19890.12479796408027224No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19620.12310387407013282No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC19620.12310387407013282No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATGCC19620.12310387407013282No Hit
GCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGG18770.11777062774191605No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18770.11777062774191605No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG18650.11701769884852076No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18510.11613928180622622No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18450.11576281735952856No Hit
GTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTG18200.11419421549828834No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA18040.11319031030709459No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAAC17910.11237463733924967No Hit
ATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAACTATAAAGCTG17740.11130798807360631No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17590.11036682695686219No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17590.11036682695686219No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17500.10980213028681572No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT17440.10942566584011806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG17230.10810804027667628No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17060.10704139101103292No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAG16950.10635120619208722No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC16940.1062884621176376No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16900.10603748581983917No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG16890.10597474174538957No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16790.10534730100089347No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16750.10509632470309505No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGC16730.10497083655419581No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT16720.10490809247974621No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16670.10459437210749818No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16620.10428065173525011No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16400.10290028209735873No Hit
CACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC16390.10283753802290913No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16020.10051600726827359No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA16020.10051600726827359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA200.004943045357.22730668
CACGCGT200.006026499454.4318867
CGTTTAG200.007477257451.54272526
CCGTTTA354.570462E-449.11263369
CGTTTAA554.8679215E-448.4866770
GTGGTAT3200.046.146121
GTATCAA29350.041.186161
CGGTTTG7200.037.96439470
TATCAAC32550.037.0986632
ATCAACG32850.036.4294133
TCAACGC33050.036.208964
CAACGCA33900.035.3032845
AACGCAG34300.034.8893936
CGCAGAG35300.033.7067538
AGAGTAC35650.033.5755711
CAGAGTA36600.032.60422510
GCAGAGT37300.031.9913449
ACGCAGA37850.031.527477
GTATATA553.346854E-431.2193641
CGTAAAC450.004442826430.91715650
Produced by FastQC (version 0.11.5)