FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.042.2901761
GTATCAA19800.036.1989971
TCAACGC20050.035.6920474
CAACGCA20200.035.5927125
ATCAACG20000.035.439523
GGTATCA9150.033.8937641
ACGCAGA21350.033.514427
AACGCAG21500.033.2805986
CGCAGAG22050.032.760378
TATCAAC21900.032.6896742
AGAGTAC22250.032.46811
TGTTAGA2058.8402885E-1031.8822670
CAGAGTA22800.031.83153310
GCAGAGT23400.030.8683479
CGGGATA1850.029.76152217
AGTACGG22850.029.35719913
GAGTACG23450.028.45935812
GTTATAC500.00783556627.5258433
TACGGGA11200.025.18942815
GTACGGG26750.024.81988314
Produced by FastQC (version 0.11.5)