FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321950
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8300.2578040068333592No Hit
GTGTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCA7760.24103121602733346No Hit
GTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7620.23668271470725266No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA6690.20779624165243052No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6100.18947041466066158No Hit
CAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5890.18294766268054044No Hit
GTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA5850.18170523373194594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5770.17922037583475695No Hit
GTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA5720.17766733964901382No Hit
CTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT5680.17642491070041932No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5310.1649324429259202No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4580.1422581146140705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4560.14163690013977326No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4520.14039447119117876No Hit
CCTCATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT4460.138530827768287No Hit
CATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA4380.136045969871098No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGTAGTCGTTACTTAGCCTGGTACC4380.136045969871098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4360.13542475539680074No Hit
GTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC4330.13449293368535487No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT4280.13293989749961174No Hit
GTATCATAGTGCTGACAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC4220.13107625407672No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT4170.12952321789097687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4160.12921261065382822No Hit
GAGTGTAAGTAGTCGTTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG4070.1264171455194906No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4040.12548532380804472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4030.1251747165708961No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4020.12486410933374746No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3950.1226898586737071No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC3840.11927317906507222No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3770.11709892840503183No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3700.11492467774499146No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3690.11461407050784284No Hit
CTGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA3670.11399285603354559No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA3570.11088678366205933No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC3570.11088678366205933No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCT3520.10933374747631619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3460.10747010405342446No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT3460.10747010405342446No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC3360.1043640316819382No Hit
CCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTC3220.10001553036185742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)