FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706606
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGG45610.6454799421459767No Hit
GTATTGTACTGTTGACAGTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC35350.5002787975194097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33370.47225752399498444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30350.4295180057910632No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCA30290.428668876290323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22830.32309377503163006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21100.2986105410936222No Hit
GTCTGTGTCTCCGGGGGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGG20340.2878549007509135No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19410.27469339348944105No Hit
CTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT19330.2735612208217875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19110.2704477459857403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18940.26804187906697646No Hit
CATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCA17520.2479458142161261No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17290.24469081779662213No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTG17070.24157734296057493No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16670.2359164796223072No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16270.23025561628403945No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15890.22487779611268513No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGC15830.22402866661194498No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15750.22289649394429145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15330.21695258743911033No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15110.21383911260306313No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAAGTGGAGGCCAGGTATTGTA14700.20803672768133868No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGG14540.2057723823460316No Hit
GTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGAC14030.19855478158974024No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14010.19827173842282686No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13750.19459217725295283No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13630.19289391825147253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAAGTGGAGG13430.19006348658233868No Hit
GTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGA13320.18850674916431504No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTA13250.1875160980801182No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13240.1873745764966615No Hit
GTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA12940.1831289289929607No Hit
GTCCCAGACCCACTGCCCCGGAATCTGCCTGGGACACCAAAGGCACTGGT12900.18256284265913394No Hit
GTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGACC12450.17619437140358277No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12400.1754867634862993No Hit
CTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTC12370.1750621987359292No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11570.16374047205939377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11190.15836265188803944No Hit
GTGCTGACGCAGTCGCCAGCCACCCTGTCTGTGTCTCCGGGGGCAAGAGC10960.1551076554685355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10840.15340939646705518No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10610.15015440004755126No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10550.1493052705468111No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT10470.14817309787915756No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10380.14689940362804732No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10350.14647483887767723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10350.14647483887767723No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10140.14350288562508667No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATT10130.14336136404163No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10030.14194614820706306No Hit
CTGCAGGAGAGGGTGGCTCTTGCCCCCGGAGACACAGACAGGGTGGCTGG9920.14038941078903944No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC9840.1392572381213859No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9760.13812506545373235No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCCCGGAATCTGCCTGGGACACCA9590.13571919853496858No Hit
CCAAAGGCACTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC9540.1350115906176851No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9380.13274724528237802No Hit
GTACTGTTGACAGTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGA9300.13161507261472447No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT9270.13119050786435438No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9230.1306244215305276No Hit
CAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG9190.13005833519670085No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9160.12963377044633076No Hit
TCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCACT9080.12850159777867723No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAATTGTTTTTA8980.1270863819441103No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTCGC8940.12652029561028352No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8790.12439747185843313No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAT8760.12397290710806305No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTCGCCAGCCACC8720.12340682077423629No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8670.12269921285695282No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8670.12269921285695282No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGCCTGGTATCAGCAGAAACCTGGCCAGG8670.12269921285695282No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8520.1205763891051024No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8420.11916117327053549No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCA8400.1188781301036221No Hit
GGTTTCTGCTGATACCAGGCTAAATTGTTTTTAATATCCTGACTGGCCCT8330.11788747901942526No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8210.11618922001794493No Hit
CCCTGTCTGTGTCTCCGGGGGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGC8080.11434943943300793No Hit
GTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAA7980.11293422359844099No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7940.11236813726461423No Hit
CCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATT7870.11137748618041737No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7850.111094443013504No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7850.111094443013504No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7760.10982074876239374No Hit
GTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGA7730.10939618401202367No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7620.10783944659400005No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7550.10684879550980321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7550.10684879550980321No Hit
GACACAGACAGGGTGGCTGGCGACTGCGTCAGCACCACATCTCCAGTGAT7530.10656575234288981No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7500.10614118759251974No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7480.10585814442560634No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACG7480.10585814442560634No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7400.1047259717579528No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7350.10401836384066934No Hit
GGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA7290.1031692343399292No Hit
CACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTT7280.10302771275647249No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7090.10033880267079531No Hit
GCCTGGGACACCAAAGGCACTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATA7090.10033880267079531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC150.002416717668.5525414
CCGATTA200.007164224552.10017846
GTGGTAT1800.045.7146341
AACACCG350.001344460439.44644539
GTATCAA14750.039.2835921
TGTACGA350.001391253839.17288210
GTACGAC350.001391737839.17011311
TATCAAC17100.034.083062
ATCAACG17200.034.0769923
CAACGCA17250.033.9782185
TCAACGC18500.031.6823924
CGCAGAG18650.031.4275728
ACAAGTT553.3809606E-431.16245516
AGAGTAC18950.030.74698811
AACGCAG19000.030.6682436
ATAGTAC450.004770932730.4677968
CATGTAG1802.3336906E-730.11193570
GTGTATA802.9032053E-629.99173713
ACGCAGA20000.029.3062117
TTCCGCT2050.028.57385837
Produced by FastQC (version 0.11.5)