FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706606
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGG45800.6481688522316539No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33070.4680118764912837No Hit
GTATTGTACTGTTGACAGTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC32350.4578223224824018No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCA28580.40446868551922854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24750.3502659190553151No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21730.3075264008513939No Hit
GTCTGTGTCTCCGGGGGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGG20840.29493097992374817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19490.27582556615709464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19430.27497643665635446No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18860.266909706399323No Hit
CTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT18780.26577753373166946No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17250.2441247314627954No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTG16880.23888843287489778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16660.2357749580388505No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16240.2298310515336694No Hit
GTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGAC15630.22119823494281113No Hit
CATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCA15230.21553737160454342No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15160.21454672052034657No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGC14900.21086715935047254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14880.21058411618355916No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAAGTGGAGGCCAGGTATTGTA14860.2103010730166458No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14760.20888585718207886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13930.1971395657551733No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13720.19416761250258277No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13600.19246935350110245No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13260.1876576196635749No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAAGTGGAGG13150.18610088224555127No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGG13140.1859593606620946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12890.18242132107567727No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12880.18227979949222056No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12870.18213827790876386No Hit
GTCCCAGACCCACTGCCCCGGAATCTGCCTGGGACACCAAAGGCACTGGT12870.18213827790876386No Hit
CTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTC12760.18058154049074024No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTA12680.1794493678230867No Hit
GTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA12360.17492067715247253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11800.16699546847889774No Hit
GTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGA11560.1635989504759371No Hit
GTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGACC11310.16006091088951976No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10960.1551076554685355No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10870.15383396121742526No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10780.15256026696631503No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10690.1512865727152048No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10420.14746548996187409No Hit
GTGCTGACGCAGTCGCCAGCCACCCTGTCTGTGTCTCCGGGGGCAAGAGC10310.14590875254385044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10230.14477657987619694No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCCCGGAATCTGCCTGGGACACCA10220.14463505829274023No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT9910.14024788920558273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9820.1389741949544725No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATT9600.13586072011842526No Hit
CAACAGTACAATACCTGGCCTCCACTTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG9590.13571919853496858No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9480.13416246111694494No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9450.13373789636657488No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9360.13246420211546464No Hit
GGTTTCTGCTGATACCAGGCTAAATTGTTTTTAATATCCTGACTGGCCCT9340.13218115894855126No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC9170.12977529202978746No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8990.127227903527567No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8920.12623725244337014No Hit
CCAAAGGCACTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC8890.12581268769300005No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8770.12411442869151973No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTCGCCAGCCACC8700.12312377760732289No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAT8690.12298225602386621No Hit
GTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAA8650.12241616969003942No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8650.12241616969003942No Hit
CTGCAGGAGAGGGTGGCTCTTGCCCCCGGAGACACAGACAGGGTGGCTGG8650.12241616969003942No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8410.1190196516870788No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAATTGTTTTTA8400.1188781301036221No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGCCTGGTATCAGCAGAAACCTGGCCAGG8400.1188781301036221No Hit
GTACTGTTGACAGTAATAAACTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGA8290.11732139268559848No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8250.11675530635177171No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8120.1149155257668347No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACGCAGTCGC8020.11350030993226778No Hit
TCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCACT7960.11265118043152761No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7950.11250965884807092No Hit
CCCTGTCTGTGTCTCCGGGGGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATATTAAAAACAATTTAGC7930.11222661568115752No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT7920.11208509409770084No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7910.11194357251424415No Hit
GTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGA7770.10996227034585045No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7750.10967922717893706No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCA7700.1089716192616536No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7680.1086885760947402No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCACTGGAGATGTGGTGCTGACG7640.10812248976091343No Hit
CACCATCAGCAGTCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAACAGTACAATACCTGGCCTCCACTTTT7630.10798096817745675No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7520.10642423075943312No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7470.10571662284214965No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7470.10571662284214965No Hit
CCTCATCTATGGTGCATCCACCAGTGCCTTTGGTGTCCCAGGCAGATTCCGGGGCAGTGGGTCTGGGACAGAATT7310.10345227750684256No Hit
GACACAGACAGGGTGGCTGGCGACTGCGTCAGCACCACATCTCCAGTGAT7300.10331075592338587No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT7290.1031692343399292No Hit
GGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA7290.1031692343399292No Hit
GCCTGGGACACCAAAGGCACTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATA7260.10274466958955909No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7090.10033880267079531No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7080.10019728108733864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATG200.007574061451.37231419
ATTCCGT200.007601679351.3250436
AGTCTAG354.4865368E-449.29269869
GTATCAA16000.042.5931971
GTGGTAT2200.042.028921
CATGTAG1955.456968E-1239.469570
TATCAAC18350.036.9178542
ATCAACG18650.036.1328773
CAACGCA18850.035.5781025
CGCAGAG19850.033.7857558
TCAACGC20100.033.7036174
AACGCAG20200.033.200366
AGAGTAC20550.032.63490311
ACGCAGA20950.032.0118037
GAGTTTA751.7671664E-631.9627341
ACTATAC553.422713E-431.0972823
TCGCACT450.004795061430.43638418
GCAGAGT23300.029.3705549
GATTCAC1401.6370905E-1129.3535291
GAGTACG21550.028.26251612
Produced by FastQC (version 0.11.5)