FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980342
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTCCAGTGGCTACTATTGGACCTGGATCCGCCAGCC31870.32509063163671453No Hit
GCTTAGGAGGCTGGCCTGATTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTCTAAAAGACT30450.31060589059736293No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26780.27316997537594023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25810.2632754691730029No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGACCTCAGTTTCAGGGAGA23960.24440450373441105No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT21320.21747512602744756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19130.1951359831568983No Hit
TTATAATACTCCGCGGACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT17820.18177329952200352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17000.17340887159787094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16100.16422840192504248No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16020.1634123601763466No Hit
CTTTTAGACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA15910.1622903027718898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15670.1598421775258022No Hit
GTTTATTACTGTGCGAGATCGCGCAGTGGCTGGCGACAGGCATACTTCCAGGACTGGGGCCAGGGCACCCTGGT15640.15953616187004127No Hit
GTCTAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGGCAGGGAGTCTGGAGA15480.15790407837264955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15350.15657801053101877No Hit
ATTATAATACTCCGCGGACTTTTGGCCAGGGGACCAAGTTGGAGATCAAA15160.1546399113778661No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15020.1532118383176483No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14830.15127373916449566No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14720.15015168176003885No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14580.14872360869982107No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14510.1480095721697122No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA14470.14760155129536426No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14390.1467855095466684No Hit
GTATTATAATATTGCTGACAATAATAAAGGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14150.14433738430058082No Hit
GTAAATGAGCAGCTTAGGAGGCTGGCCTGATTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGT13680.1395431390269926No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13680.1395431390269926No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13090.13352483113036062No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12930.1318927476329689No Hit
GTCCTGGAAGTATGCCTGTCGCCAGCCACTGCGCGATCTCGCACAGTAAT12510.12760852845231563No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12510.12760852845231563No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12510.12760852845231563No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12380.12628246061068485No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGGCCTGATTTCTGCTGGTA12320.12567042929916294No Hit
GTCATGGACCTCCTGCACAAGAACATGGGACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12170.1241403510203582No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11870.12108019446274872No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11720.11955011618394397No Hit
ATAATACTCCGCGGACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGA11510.11740800659361733No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11440.11669397006350846No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11310.11536790222187766No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11240.11465386569176879No Hit
GTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTAGA11230.1145518604731818No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11150.11373581872448593No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10830.11047165172970248No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10820.1103696465111155No Hit
CTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTAGACAACTCCAA10630.10843154735796284No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10560.10771751082785394No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAATAGTCCTGCGCCCCACTGCTGTAGTTGCAC10490.10700347429774507No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTC10450.10659545342339713No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10210.10414732817730955No Hit
CAATAATAAAGGGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10180.1038413125215486No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10150.10353529686578766No Hit
CTATTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATGAATCGTAGTGGAAGCCC10040.10241323946133085No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10000.10200521858698292No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC9810.10006711943383023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG252.597039E-455.0701135
TCGTATG950.054.5495940
TACTAAT7450.051.76207470
GGTATCA6800.042.828171
CTCGTAT1300.042.5010339
GTATCAA17700.041.6183361
GTGGTAT2300.040.221241
AATAGCG654.3734144E-738.2120862
TATCAAC19650.038.007492
TATACAC1450.037.7992253
TGGTATC2500.036.9997632
CTTATAC1500.036.5467031
TAAGACA1800.036.2418231
CGTATGC1350.035.8589941
ATCTCGT1450.035.63855437
ACGAGAC1550.035.3731922
ATGCCGT1400.034.6747244
CGCGGTA400.002548040134.6318143
ATCAACG21500.034.5742033
TGGATTG11550.034.51944470
Produced by FastQC (version 0.11.5)