FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381352
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43300.31346101500558876No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34180.2474387411753123No Hit
CAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA33220.24048902814054637No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31610.228833780238491No Hit
CTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA31520.22818224464148168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30970.22420063821531366No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG30630.22173928151550076No Hit
GTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29050.21030121214578182No Hit
GGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC28500.20631960571961383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26350.1907551442355026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25420.1840226097330731No Hit
GTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA24260.17562503981606428No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23530.17034036219587767No Hit
GTAATATATAGCCGTGTCGTCAGCTCTCAGCCTGCTCACTTGCAGATACA23390.16932686237830763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23150.16758943411961613No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22980.16635875576970968No Hit
CTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA22880.16563482732858822No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22700.1643317561345696No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22530.16310107778466315No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21820.15796118585270083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21810.15788879300858868No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21320.15434154364709357No Hit
GTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCT21040.15231454401195352No Hit
ATATATTACTGTGCGAAAGATCATGACATTCGGGGAGTTGTTTACGACTT20980.15188018694728064No Hit
GTCAGGGACCCCGGCGGCCCGCTTAGAACCCAAACAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA20870.15108386566204704No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20710.1499255801562527No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20600.14912925887101913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20330.1471746520799912No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20030.14500286675662685No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA19960.14449611684784183No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19950.1444237240037297No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCG19580.14174518877158032No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19450.14080408179812243No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGA19430.14065929610989814No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19100.13827033225419733No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18970.13732922528073946No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG17950.12994515518130065No Hit
GACTAGACCTGCAGGTGAGGGAGGCCGGCTCTCCAGCGGTGACGGGCAGG17410.1260359415992448No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG17390.1258911559110205No Hit
GTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT17280.1250948346257869No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT17250.12487765609345049No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC17030.12328501352298327No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC16880.1221991208613011No Hit
GTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTTCAGTGGCAGT16690.12082365682317034No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACT16400.11872426434391813No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16400.11872426434391813No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16210.11734880030578737No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16140.11684205039700235No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT15980.11568376489120803No Hit
TCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCA15930.11532180067064732No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15780.11423590800896515No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCC15780.11423590800896515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15770.11416351516485299No Hit
CTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGC15590.11286044397083438No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15410.11155737277681575No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT15140.10960276598578785No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG14880.10772055203887206No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14730.10663465937718987No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14340.10381133845681623No Hit
GTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGC14070.1018567316657883No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13990.10127758891289114No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13970.10113280322466686No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGC13920.10077083900410612No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13890.1005536604717697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.041.9487721
GTGGTAT4100.041.5880931
TGGTATC4300.040.4439132
GTATCAA27250.040.42211
ACCGATC1400.036.56326727
TTAGTCG400.001986264536.4361566
TATCAAC31800.034.743162
TCGTATG1102.910383E-1134.48432540
CGTATGC1350.033.24852441
ATCAACG33550.033.0289763
TCAACGC33950.032.6398284
CAACGCA34050.032.543975
CGATCAG1600.032.0125829
AACGCAG35050.031.610896
GTTATTA1053.359617E-431.19622270
CTTATAC2000.030.6920131
ACGAGAC2000.030.68756922
GTATAAC450.004892796330.31311
GGCCCGG10650.030.14170570
AGAGTAC36950.030.07660511
Produced by FastQC (version 0.11.5)