FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381352
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42620.30853830160596285No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG31260.22630003069456592No Hit
CAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA30630.22173928151550076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29840.21602024683064128No Hit
CTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA29570.21406564003961337No Hit
GGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC29450.21319692591026765No Hit
GTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29290.21203864040447332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28540.2066091770960624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27310.19770485727026854No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26420.19126189414428763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25950.1878594304710168No Hit
GTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA24200.17519068275139138No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21930.1587575071379344No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21440.1552102577764393No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21270.15397957942653284No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21140.15303847245307495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21050.15238693685606566No Hit
CTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA21020.15216975832372923No Hit
GTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCT20990.15195257979139276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20770.15035993722092558No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20650.14949122309157983No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA20470.14818815189756124No Hit
ATATATTACTGTGCGAAAGATCATGACATTCGGGGAGTTGTTTACGACTT20340.14724704492410334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20290.14688508070354261No Hit
GTAATATATAGCCGTGTCGTCAGCTCTCAGCCTGCTCACTTGCAGATACA20280.14681268785943047No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20220.1463783307947576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20010.14485808106840253No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19860.14377218840672037No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19540.14145561739513174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19250.1393562249158795No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19170.13877708216298237No Hit
GTCAGGGACCCCGGCGGCCCGCTTAGAACCCAAACAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18980.1374016181248516No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18670.1351574399573751No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18630.1348678685809265No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCG18310.13255129756933787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17760.12856969114316988No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA17740.1284249054549456No Hit
GTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT17550.12704944141681485No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGA17430.1261807272874691No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17230.12473287040522618No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG17000.12306783499064684No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT16850.12198194232896466No Hit
GTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTTCAGTGGCAGT16800.12161997810840393No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16570.1199549426938246No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC16500.11944819278503957No Hit
GACTAGACCTGCAGGTGAGGGAGGCCGGCTCTCCAGCGGTGACGGGCAGG16300.11800033590279668No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACT16150.11691444324111451No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.11249847975027365No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG15350.11112301571214289No Hit
CTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTA15180.10989233736223641No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG15050.10895123038877853No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14970.10837208763588137No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCC14910.1079377305712085No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14780.10699662359775061No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14720.10656226653307775No Hit
TCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCA14670.10620030231251701No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14510.10504201680672269No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14430.10446287405382552No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT14360.1039561241450405No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14320.10366655276859192No Hit
CTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGC14110.10214630304223689No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14110.10214630304223689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGT200.007767740651.0478411
GTGGTAT3100.042.8764041
GGTATCA12000.041.7495271
GTATCAA29350.041.3387531
TGGTATC3850.037.140492
TATCAAC33250.036.554062
ATCAACG34650.034.869253
TCAACGC34900.034.714484
CAACGCA36000.033.7470745
AACGCAG36350.033.422146
GGCCCGG9600.033.09406770
CGCAGAG38150.031.7548548
AGAGTAC38500.031.3800611
CAGAGTA39000.030.97550610
AACGTTG900.005110097630.04284170
CCCGAAA753.8023514E-529.62694767
ACGCAGA41300.029.4152727
GTAATAT5050.029.0196741
ATAGCCG4850.028.065518
AGTACGG38350.027.68796513
Produced by FastQC (version 0.11.5)