FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951348
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT47950.5040216618944907No Hit
CTATTATACTGTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC42360.44526293217623836No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCATCTA33650.35370863238268224No Hit
GTAATACAGAGCCGCGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA32070.3371006193317272No Hit
CAGTAATACAGAGCCGCGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA29820.313449967835114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27330.287276580178862No Hit
CTACTAATACTGTGACTGGCCCGGCAAGTGATAGTGACT27320.2871714661722104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26430.27781631958021674No Hit
GTATTACTGTGCGAGGTCTGGTCCAGCTGCTATCATCGACTTCTACTATTGTATGGACGTCTGGGGCCAAGGGAC25980.27308618928089406No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23960.251853159937268No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20870.21937293188191911No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG20770.21832179181540295No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19850.20865130320345446No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19830.20844107519015126No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18300.19235863217245425No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18170.19099215008598325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18150.19078192207268005No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16740.17596084713480242No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16730.17585573312815078No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16720.17575061912149917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.17396368100842172No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15770.1657647884895958No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15690.1649238764363829No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15430.16219091226344093No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15320.16103465819027316No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15230.16008863213040864No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14950.15714543994416344No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGT14660.15409713375126663No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACTATCACTTGCCG14480.15220508163153756No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14390.15125905557167302No Hit
CTGTATTACTGTGCGAGGTCTGGTCCAGCTGCTATCATCGACTTCTACTA14330.15062837153176334No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14090.1481056353721246No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13570.14263970702624065No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13420.14106299692646646No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13320.14001185685995032No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13160.13833003275352446No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13160.13833003275352446No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12100.12718794804845335No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12080.12697772003515012No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12080.12697772003515012No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT12050.12666237801519528No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC12040.12655726400854367No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12040.12655726400854367No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11950.12561123794867915No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11900.12508566791542106No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11870.12477032589546622No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGTCCAGATGACCCAGTCTCCTTCCACC11730.12329872980234363No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC11700.12298338778238879No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11680.12277315976908555No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11500.12088110764935649No Hit
CTATAATAGATACTCCCAATCCACTCCAGGGCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAATTACTA11420.12004019559614357No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11420.12004019559614357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11380.11961973956953711No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11260.11835837148971774No Hit
CAGTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11200.11772768744980806No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11160.11730723142320161No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11160.11730723142320161No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11110.11678166138994353No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTAAAGAGTCGAGTCACCATATCCGTGGACACGTCCAAGAA11100.11667654738329192No Hit
GGGTGGAAGGAGACTGGGTCATCTGGACGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG11090.11657143337664029No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11090.11657143337664029No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11050.11615097735003385No Hit
GTCTGCATCTATAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCACA10730.11278732913718217No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTG10720.11268221513053057No Hit
ACTACAACCCGTCCCTAAAGAGTCGAGTCACCATATCCGTGGACACGTCC10680.11226175910392412No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10590.11131573304405959No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA10480.11015947897089183No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10180.10700605877134339No Hit
CAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCAT10150.10669071675138855No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10140.10658560274473694No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAATTTATTACTGCCAACAGTATAATAGTTAC10130.10648048873808531No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC10020.10532423466491758No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9860.10364241055849173No Hit
TTCTAAAGTAGATGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT9860.10364241055849173No Hit
ATAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCACAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA9770.1026963844986272No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9670.10164524443211106No Hit
CAATAGTAGAAGTCGATGATAGCAGCTGGACCAGACCTCGCACAGTAATA9570.10059410436559493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGT100.008438174136.7952470
ACCGTAG158.935451E-488.0833869
AATGTCT200.00646787953.4661557
GTGGTAT1550.052.457431
TGCGAAT400.00761584151.29821470
TCGTATG1350.051.15084540
CTTATAC1550.048.085981
GTATGCC1600.047.59934642
CGTATGC1600.047.5329541
AGACATC1600.046.93120633
ACGAGAC1650.045.1835622
TATACAC1750.044.517013
AAGGGGG2000.044.0416969
ATCTCCG1700.043.82927310
GACCCTA1800.043.3647626
ACCCTAA1750.042.7094527
CACGGAT10900.042.67007470
TTATACA1750.042.5814862
GTACATT405.5673572E-542.348451
ATACACA2000.042.3395464
Produced by FastQC (version 0.11.5)