FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737767
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG49980.677449655514546No Hit
CCTATGAACAGTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25210.3417067990300461No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22880.3101250123684036No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCACCACTACTGTCCCACAC22510.3051098788641943No Hit
GTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT22190.300772466103797No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18880.2559073528634379No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCACCA18720.2537386464832393No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC17840.24181076139214686No Hit
CCCCTGTGCTGGTCGTCTTTGATGATAGCGACCGGCCCTCAGGGATCCCT16040.21741281461491227No Hit
GTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTC15700.2128043135569902No Hit
CTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGAC15680.21253322525946536No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15430.20914462154040503No Hit
GTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGACCCTGC15040.2038583997386709No Hit
AGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGT14880.20168969335847223No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACGCTTCTCCCT14780.2003342518708481No Hit
GTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTA14150.19179497049881603No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14010.18989735241614222No Hit
CCCCAGGACAGACGGCCAGCATTCCCTGTGGGGGAAACGGCATTGGAGGG13870.18799973433346842No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13850.1877286460359436No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAAAGA13750.18637320454831946No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTA13720.1859665721020322No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13400.18162915934163495No Hit
CACCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGACCCTGCTGATGGTCAGGGTGG12700.17214106892826597No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12620.17105671573816666No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12560.17024345084559217No Hit
GTGTACGCTTCTCCCTCCAATGCCGTTTCCCCCACAGGGAATGCTGGCCG12480.16915909765549286No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12370.1676681120191063No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12350.16739702372158147No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12260.16617712638271975No Hit
GTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG12260.16617712638271975No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12030.1630596109611842No Hit
GGACAGTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCC11890.16116199287851043No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11830.16034872798593594No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGCCAC11800.1599420955396487No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11680.15831556575449973No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11520.1561468593743011No Hit
CGGCCACCCTGACCATCAGCAGGGTCGCAGCCGGGGATGAGGCCGACTAT11440.1550625061842018No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11260.15262271150647833No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA10990.14896301948989316No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10870.14733648970474417No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10840.14692985725845695No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10820.1466587689609321No Hit
GTAATAAACGGCCGTGTCCGCGGCCGTCACAGAACTCAGCTTCAGGGAGAAGTGGTTGTTAGACGTGTCTACTGA10600.143676797688159No Hit
CCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGC10560.14313462109310934No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGCCACCCTCGGTGTCA10490.14218581205177244No Hit
GCATTCCCTGTGGGGGAAACGGCATTGGAGGGAGAAGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCC10350.14028819396909864No Hit
GTGTGGGACAGTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGG10270.13920384077899933No Hit
GGCTCTGTGACCTCCTATGTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTCC9850.13351098653097795No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9810.13296880993592827No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTTTGATGATAGCGACCGGCCCTCAGG9680.1312067360020169No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9660.13093564770449206No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGC9520.12903802962181826No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9460.12822476472924377No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8950.12131201314236066No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8920.12090538069607343No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGC8880.12036320410102376No Hit
GTCCCTCCACCGAACACCACATGATCACCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGG8840.11982102750597412No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8710.11805895357206272No Hit
GCATTGGAGGGAGAAGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCC8600.11656796793567618No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACAGTC8430.11426371740671512No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCAC8320.11277273177032858No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8200.1111462019851796No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8180.11087511368765476No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8110.10992630464631789No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG7980.1081642307124065No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7860.10653770092725753No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7850.1064021567784951No Hit
CCACATGATCACCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCA7760.10518225943963339No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTCCCTGTGGGGGAAACGGCATTGGAGGGAGAAGCGTA7740.10491117114210856No Hit
CAATAATAGTCGGCCTCATCCCCGGCTGCGACCCTGCTGATGGTCAGGGT7690.10423345039829647No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7600.10301355305943474No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACAGTCTGTAGGGGC7590.10287800891067235No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC7540.10220028816686026No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGCATTCCCTGTGGGGGAAACGG7510.10179365572057303No Hit
GAGTTGGAGCCAGAGAATCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAAAGACGACCAGCACAGGGGC7400.10030267008418647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)