FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737767
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG45600.6180813183566085No Hit
CCTATGAACAGTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22170.3005013778062722No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21880.2965705974921622No Hit
GTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT20390.2763745193265625No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCACCACTACTGTCCCACAC20340.27569679858275037No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17750.24059086405328511No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAAAGA16490.22351230130922095No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCACCA15980.2165995497223378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15170.20562047367258227No Hit
CCCCTGTGCTGGTCGTCTTTGATGATAGCGACCGGCCCTCAGGGATCCCT15040.2038583997386709No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC14890.2018252375072347No Hit
AGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGT14740.19979207527579845No Hit
GTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTC14030.19016844071366706No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13970.18935517582109257No Hit
CTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGAC13530.18339123327554635No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACGCTTCTCCCT13100.17756283487876254No Hit
GTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTA12880.17458086360598943No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12780.17322542211836528No Hit
GTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGACCCTGC12740.17268324552331563No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTA12610.17092117158940423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12460.16888800935796802No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12420.16834583276291837No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12180.16509277319262042No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12080.1637373317049963No Hit
GTGTACGCTTCTCCCTCCAATGCCGTTTCCCCCACAGGGAATGCTGGCCG11900.16129753702727284No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGC11740.15912883064707423No Hit
CCCCAGGACAGACGGCCAGCATTCCCTGTGGGGGAAACGGCATTGGAGGG11690.15845110990326214No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11600.1572312125644004No Hit
GTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG11480.15560468277925144No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11290.15302934395276557No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11130.15086063757256696No Hit
CACCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGCGACCCTGCTGATGGTCAGGGTGG11130.15086063757256696No Hit
GGACAGTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCC10980.14882747534113075No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGCCAC10950.1484208428948435No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10890.147607578002269No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10850.14706540140721935No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA10760.14584550406835764No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10420.14123700301043554No Hit
GTAATAAACGGCCGTGTCCGCGGCCGTCACAGAACTCAGCTTCAGGGAGAAGTGGTTGTTAGACGTGTCTACTGA10340.14015264982033623No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10340.14015264982033623No Hit
CGGCCACCCTGACCATCAGCAGGGTCGCAGCCGGGGATGAGGCCGACTAT10290.13947492907652415No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10290.13947492907652415No Hit
GTGTGGGACAGTAGTGGTGATCATGTGGTGTTCGGTGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGG10260.13906829663023693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9980.13527306046488932No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9860.13364653067974033No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9830.1332398982334531No Hit
GGCTCTGTGACCTCCTATGTACTGTCTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTCC9820.1331043540846907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9810.13296880993592827No Hit
CCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTGC9510.12890248547305586No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC9290.12592051420028275No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTTTGATGATAGCGACCGGCCCTCAGG9240.12524279345647069No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGTCTCAGCCACCCTCGGTGTCA9210.12483616101018344No Hit
GCATTCCCTGTGGGGGAAACGGCATTGGAGGGAGAAGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCC8810.11941439505968687No Hit
GTCCCTCCACCGAACACCACATGATCACCACTACTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGG8630.11697460038196342No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8400.11385708496042789No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8260.1119594668777541No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8000.10843531900993132No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8000.10843531900993132No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACAGTC7970.10802868656364407No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7960.10789314241488165No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTCCCTGTGGGGGAAACGGCATTGGAGGGAGAAGCGTA7950.10775759826611925No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7850.1064021567784951No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7840.10626661262973268No Hit
GCATTGGAGGGAGAAGCGTACACTGGTACCAGCAGAAGCCAGGCCAGGCC7760.10518225943963339No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCACCGAACACCACATGATCAC7670.10396236210077166No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7550.10233583231562268No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGC7540.10220028816686026No Hit
CTATTATTGTCAGGTGTGGGACAGTAGTGGTGATCATGTGGTGTTCGGTG7520.10192919986933545No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7440.10084484667923611No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7430.10070930253047372No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG7420.1005737583817113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)