FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314488
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATATA38160.2903031446464327No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT38040.28939024167584637No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33930.2581233149332668No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29960.22792144165637113No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29230.2223679485853047No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29210.22221579809020697No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27400.20844617828386414No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24880.18927521590155255No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24860.18912306540645482No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24520.18653650698979374No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT23630.17976580995794558No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22860.17390801589668373No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGCACCCAGGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG22630.17215828520306006No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22190.1688109743109104No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG22180.16873489906336156No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC21920.16675694262709131No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21610.16439860995307679No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21090.1604426970805363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20620.1568671604457401No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGAATTCACCTTTAGCACCCAGGCCATGAGCTGGGTCCG20320.15458490301927444No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCCTGGGTGC19750.15024861390898966No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19610.14918356044330566No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGCGAGGCGCAGCTGTTGGAGT19240.14636877628399803No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19190.14598840004625377No Hit
GAGTTTGTAGAGCTTGCATGCAGAAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT19040.14484727133302092No Hit
CTACAAACTCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG18760.14271716440165297No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18180.13830480004381934No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT18180.13830480004381934No Hit
GTTTATTACTGTGCGAGAGTCTTTCAACATAGGGGAACAAGGTTCTACTA18060.13739189707323307No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18040.13723974657813537No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17940.1364789941026468No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17930.13640291885509798No Hit
GCCCTGGGATTTTCAGGTGATTTCATCTGGTGATCAGGACTGAACAGAGA17830.1356421663796094No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17790.13533786538941397No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17480.13297953271539945No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGATTTCATCTGGTGAT17210.13092550103158035No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16830.12803464162472383No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16700.12704566340658874No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG16450.12514378221786734No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16400.12476340598012306No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16310.12407872875218336No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16290.12392657825708564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15900.12095964360268029No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15900.12095964360268029No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15900.12095964360268029No Hit
TCCCCGGAGTTTGTAGAGCTTGCATGCAGAAATAAACCCCAACATCCTCA15840.12050319211738714No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15750.11981851488944745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15720.11959028914680088No Hit
GTATTAGTACTAGTGGTGGTGAGACATACTACGCAAACTCTATTAAGGGCCGGTTCAGCGTCTCCAGAGACAATT15480.11776448320562835No Hit
CTCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15400.1171558812252375No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC15320.11654727924484667No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15320.11654727924484667No Hit
GTACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC15110.1149496990463207No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA15080.11472147330367413No Hit
GGGCTGGAGTGGGTCTCAGGTATTAGTACTAGTGGTGGTGAGACATACTA14680.1116784634017199No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14670.11160238815417106No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA14590.11099378617378021No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14530.11053733468848709No Hit
GTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14530.11053733468848709No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14420.10970050696544967No Hit
GTATGTCTCACCACCACTAGTACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT14420.10970050696544967No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA14350.10916798023260768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14230.10825507726202141No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14070.10703787330123972No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14060.10696179805369088No Hit
CCTTTAGCACCCAGGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGGTATTAGTA14040.10680964755859315No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13950.10612497033065345No Hit
GGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT13920.10589674458800688No Hit
GCCCAGCCCTGGGATTTTCAGGTGATTTCATCTGGTGATCAGGACTGAAC13910.10582066934045804No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA13830.10521206736006719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13820.10513599211251833No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13810.10505991686496949No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13740.1045273901321275No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13710.10429916438948093No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC13670.10399486339928549No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT13650.10384271290418778No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13650.10384271290418778No Hit
CTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGATTTCATCTGGTGATCAGGACTGAACAGAGAGAACTCA13610.10353841191399236No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13600.1034623366664435No Hit
GGTCTAGTCAGAGCCTCCTGCATAGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC13590.10338626141889466No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13540.10300588518115039No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13460.10239728320075954No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13370.10171260597281985No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13370.10171260597281985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13280.10102792874488013No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13220.10057147725958701No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13200.10041932676448931No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13170.10019110102184273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.049.552371
CGTATGC901.8189894E-1242.2656941
GTATCAA20700.041.6380161
TCGTATG955.456968E-1240.0105240
GTGGTAT1850.037.1231541
TATCAAC23250.037.067032
ACGAGAC1051.6370905E-1135.9722
GTGTATA1350.035.6012513
ATCAACG25150.034.1276253
TCAACGC25150.034.1276254
CAACGCA25350.033.9938055
AACGCAG25700.033.6631626
TGTATAA1450.033.1472514
ATGCCGT1154.5474735E-1133.13717744
CGCAGAG27300.031.5632728
CTTATAC1208.185452E-1131.4773431
AGAGTAC27350.031.3800511
CAGAGTA27850.030.93994310
GCTAATC902.0406151E-730.5886633
TCTGGAG19500.030.49486470
Produced by FastQC (version 0.11.5)