FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314488
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATATA37190.2829238456341937No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT36040.27417519216607533No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35310.2686216990950088No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28720.21848811096031304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28220.2146843485828703No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26320.20023005154858772No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25620.19490478422016785No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23880.18166769114666698No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT22200.16888704955845926No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22090.16805022183542184No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21450.1631814059922951No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21340.1623445782692577No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGCACCCAGGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG21050.1601383960903409No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20320.15458490301927444No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC19930.15161796836486904No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCCTGGGTGC19850.15100936638447823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19750.15024861390898966No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19530.14857495846291485No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19370.14735775450213315No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19350.14720560400703545No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGAATTCACCTTTAGCACCCAGGCCATGAGCTGGGTCCG19290.1467491525217423No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT18760.14271716440165297No Hit
GAGTTTGTAGAGCTTGCATGCAGAAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT18710.14233678816390868No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18130.13792442380607509No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGCGAGGCGCAGCTGTTGGAGT18050.13731582182568422No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG17870.1359464673698048No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17660.13434888717127885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17360.1320666297448132No Hit
CTACAAACTCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG17280.13145802776442234No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17100.13008867330854293No Hit
GCCCTGGGATTTTCAGGTGATTTCATCTGGTGATCAGGACTGAACAGAGA17100.13008867330854293No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16990.12925184558550554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16880.12841501786246812No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16700.12704566340658874No Hit
GTTTATTACTGTGCGAGAGTCTTTCAACATAGGGGAACAAGGTTCTACTA16600.12628491093110017No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16490.12544808320806275No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16380.12461125548502536No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16280.1238505030095368No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16230.12347012677179252No Hit
GTATTAGTACTAGTGGTGGTGAGACATACTACGCAAACTCTATTAAGGGCCGGTTCAGCGTCTCCAGAGACAATT15950.12134001984042456No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15620.11882953667131232No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15550.11829700993847034No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT15540.11822093469092149No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15430.11738410696788407No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15390.11707980597768866No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG15270.11616690300710239No Hit
GGGCTGGAGTGGGTCTCAGGTATTAGTACTAGTGGTGGTGAGACATACTA15180.11548222577916269No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15060.11456932280857642No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGATTTCATCTGGTGAT14920.11350426934289244No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14900.11335211884779474No Hit
GTACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC14890.11327604360024587No Hit
GTATGTCTCACCACCACTAGTACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT14860.1130478178575993No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14820.11274351686740389No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14790.11251529112475732No Hit
TCCCCGGAGTTTGTAGAGCTTGCATGCAGAAATAAACCCCAACATCCTCA14740.11213491488701305No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA14670.11160238815417106No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14480.1101569584507428No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14350.10916798023260768No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14300.1087876039948634No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14190.10795077627182598No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14160.10772255052917944No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA14140.1075704000340817No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14040.10680964755859315No Hit
CTCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13980.10635319607330003No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13800.10498384161742062No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC13740.1045273901321275No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGG13710.10429916438948093No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13670.10399486339928549No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13640.10376663765663895No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT13520.10285373468605268No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13280.10102792874488013No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13160.10011502577429386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2650.050.479712
GTGGTAT2900.047.3486331
GGTATCA9200.045.1484641
GTATCAA23050.040.2103921
TATCAAC24700.037.216542
TCTGGAG17650.035.9158270
ATCAACG26900.034.2807433
TCAACGC27050.034.214774
AACGCAG27550.033.4732136
CAACGCA27700.033.415715
CGCAGAG29150.031.3995118
AGAGTAC29250.031.29335211
CAGAGTA29700.030.70261810
GCATAAG500.00792654527.4622061
ACCTATT500.00796345127.43607517
GTACAAT500.0079782527.4256366
CGCGCAC657.880961E-426.96902356
AGTACGG30600.026.10563713
GAGTACG30850.025.89408312
ACGCAGA36800.025.0594317
Produced by FastQC (version 0.11.5)