FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382635
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTA250.00804359850.58979869
GTATCAA16600.039.153351
GTCTTCG350.001440914938.889791
CGTATGC557.0037277E-637.925341
TCGTATG557.0941223E-637.85451540
TATCAAC19050.034.470632
TCAACGC19300.034.024124
ATCAACG19500.033.675153
CAACGCA19400.033.6733555
GGTATCA7000.033.0563241
AACGCAG19950.032.7364586
TGGACTA800.004932490630.2515770
ACGCAGA21600.030.2357567
ACGGGAC1151.9699655E-929.58232116
TACGGGA5550.029.41857315
AGAGTAC22300.029.2866511
CGCAGAG22350.029.2211328
CTTATAC704.2226933E-529.1673431
GCAGAGT22650.028.9842769
CAGAGTA22700.028.77058610
Produced by FastQC (version 0.11.5)