FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382635
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7540.19705463431207285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG200.007776085851.0268752
GTATCAA16250.037.5211071
GGTATCA7250.036.1766781
TATCAAC18450.033.5569652
ATCAACG18300.032.6953053
TCAACGC18500.032.346074
CAACGCA18850.031.749635
AACGCAG18900.031.6656386
ACGCAGA19400.030.6742297
CAGAGTA20050.029.67980410
CGCAGAG20450.028.932998
AGAGTAC20550.028.79972611
TACGGGG5600.028.54701815
GGGGCGA500.006799699328.33328657
GCAGAGT20850.028.2148279
CGTTAAC500.007559099727.72555239
AGCCCGT500.00828310427.21077311
TAGTGGC500.00829367727.203667
CTATACA500.00829896927.2001044
GAGTACG16700.027.0885712
Produced by FastQC (version 0.11.5)