FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395894
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7520.18994983505685867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA150.002327017569.20373541
ATTACGT252.4741772E-455.6046245
CCGTCAT400.00960233248.3667870
CGGGTCG306.555352E-445.63998818
GTATCAA13150.043.7921
CGACTGG650.001303016639.68556670
CAACGCA14600.039.1238025
ATCAACG14550.039.0231673
TCAACGC14600.038.8846174
TATCAAC15400.037.327652
GGTATCA4800.037.1341361
AACGCAG15600.036.8351256
ACGGGTA853.1996024E-936.23893716
AGAGTAC16050.036.2331611
CGCAGAG16100.036.116078
CAGAGTA16300.035.67292810
ATACGAG400.002349228635.2048156
GCAGAGT16800.034.4076429
ACGCAGA16850.034.305547
AGTACGG15900.032.91748413
Produced by FastQC (version 0.11.5)