FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370950
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26630.7178865076155816No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11840.31918048254481735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8740.23561126836500876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8670.23372422159320663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8060.21727995686750237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7790.21000134789055128No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7470.20137484836231298No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7450.20083569214179808No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7370.1986790672597385No Hit
GCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT7150.19274834883407468No Hit
CTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC6990.18843509906995554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6910.18627847418789595No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC6760.18223480253403423No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6730.1814260682032619No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6460.1741474592263108No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA6440.1736083030057959No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT6430.17333872489553848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6410.17279956867502358No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6380.17199083434425125No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6240.16821674080064697No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6220.16767758458013207No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5920.15959024127240867No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT5920.15959024127240867No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5780.15581614772880442No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT5720.15419867906725976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5550.14961585119288315No Hit
GATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA5480.14772880442108102No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC5350.1442242889877342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5240.1412589297749023No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTTATAGTTTT5210.14045019544412993No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTA5190.13991103922361506No Hit
CTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTT5180.1396414611133576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5030.13559778945949588No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5000.13478905512872355No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4930.13290200835692143No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4730.12751044615177248No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC4710.12697128993125759No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC4690.1264321337107427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4520.12184930583636609No Hit
ACTTATTCCTGTCAACAGCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA4500.1213101496158512No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4480.1207709933953363No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4350.11726647796198948No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG4350.11726647796198948No Hit
ATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA4250.11457069685941502No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4200.11322280630812777No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4170.11241407197735544No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC4120.11106618142606821No Hit
ACCTTGATGGGACCCCATTTTGTAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG3990.10756166599272139No Hit
GGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCA3960.10675293166194905No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3930.1059441973311767No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3830.10324841622860224No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3760.1013613694568001No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC3760.1013613694568001No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3740.10082221323628521No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG3730.10055263512602777No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTGTAAAGT3720.10028305701577032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAT150.002731640666.4605223
ACGAATA150.002731640666.4605224
GTTCACG451.1448663E-464.96078570
CGAATAG200.0085029549.8858625
CGACGAA200.00853939349.83191322
CCGACGA200.00855308949.81171821
ATCATTA359.337751E-442.4740959
GTATCAA8450.039.6546671
GCGTGCC853.6852725E-935.71618335
TATCAAC9400.035.618172
ATCAACG9950.033.635713
TCAACGC10200.033.1317064
CAACGCA10300.032.810045
AACGCAG10600.032.1940166
CGCGTGC951.1146767E-831.88656234
AGAGTAC10750.031.75335911
TGGCGCG951.178887E-831.70420331
CTCGGTG450.00444103930.91313444
GTGGTAT1400.030.8066861
ACGGGCC1309.094947E-1230.63266816
Produced by FastQC (version 0.11.5)