FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844973
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25250.041.3157351
GGTATCA9500.039.012471
ATCAACG27900.037.507823
CAACGCA28050.037.3072435
TCAACGC28050.037.3072434
TATCAAC28150.036.9353182
AACGCAG30100.034.880376
CGTATAG400.00255368334.61564339
ACGCAGA31650.033.1721737
TCGTATG1056.1299943E-1033.0007740
AGAGTAC31700.032.90337811
CGCAGAG32600.032.3107458
CAGAGTA32450.032.14290210
GCAGAGT34250.030.5538169
AGTACGG26850.029.90359913
GAGTACG27100.029.62598212
GTGGTAT3300.029.1170521
CTCGTAT1202.588422E-928.8463739
GTACGGG29650.027.08125514
CGTATGC1305.9517333E-926.68157841
Produced by FastQC (version 0.11.5)