FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844973
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12060.14272645398136982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21300.036.7394681
GGTATCA10400.035.6424941
CGGCGTA751.5402129E-632.53161247
TATCAAC25150.030.9513532
ATCAACG25600.030.6642993
TCAACGC25650.030.6009034
CAACGCA25950.030.115055
AACGCAG27000.028.943916
CGCAGAG28500.027.5408128
ACGCAGA28450.027.4687377
AGAGTAC29400.026.69930511
CAGAGTA29550.026.67977710
GCAGAGT31900.024.927779
AGTACGG24850.024.69098713
GTGGTAT4350.024.4596421
GAGTACG25550.024.14868212
GTGTAAT1052.3726418E-522.8816011
CGATAGG750.002068895622.85180912
TACGGGA9300.022.1185815
GTACGGG27850.021.78511614
Produced by FastQC (version 0.11.5)