FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861212
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1150.042.1604539
GGTATCA10850.038.5994151
TCGTATG1300.037.33514440
GACTAAG1750.037.29656626
GTATCAA24300.037.2947961
GTGGTAT4250.033.1165851
ACTAAGG2050.031.84593427
CGTATGC1550.031.34275241
AGACTAA2250.030.52278725
TCAACGC29450.030.4214974
ATCAACG29600.030.3832993
TATCAAC29850.030.3605862
CAACGCA29950.029.9136285
TGGTATC4600.029.850582
AACGCAG31200.028.6051456
TTTACCG500.00783296427.52806530
ACGCAGA33000.027.0448637
AGAGTAC33800.026.50630411
ACGAGAC2600.026.41088322
AATCTCG1850.026.15881336
Produced by FastQC (version 0.11.5)