FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861212
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16190.1879908779719744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.041.1945951
GTATCAA27600.037.935841
GTGGTAT3100.034.3288271
CAACGCA30550.033.8980945
ATCAACG30800.033.622953
TCAACGC30850.033.56654
AACGCAG31100.033.298616
TATCAAC31400.033.2085272
ACGCAGA33550.030.9673777
GGGTATA450.0047358830.5145151
AGAGTAC34800.029.56288111
CGCAGAG35200.029.418378
CAGAGTA35000.029.39053710
AGTACGG30650.028.19845213
GCAGAGT37350.027.8167489
GAGTACG31250.027.5472912
TACGGGC9050.026.90854315
CTACGCT658.9710223E-426.3761254
GCGTAGA1501.0858265E-425.39071370
GTACGGG34000.025.31920214
Produced by FastQC (version 0.11.5)