FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369832
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16790.12256977497970553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.042.1228751
CTCGTAT3750.041.47009339
TCGTATG3850.040.43621440
TCTCGTA4050.039.20234338
GTATCAA41750.038.7449531
TATCAAC46450.034.8220372
AGGGGGG7150.034.7449570
ATCAACG46550.034.5268483
TCAACGC46550.034.5230674
ACGAGAC5250.034.5169122
CGTATGC4650.034.26267241
CAACGCA48250.033.4484375
AACGCAG49950.032.2357186
CTTATAC5900.031.880211
AATCTCG5250.030.18285836
AGAGTAC53350.030.11725211
ACGCAGA53500.030.0967127
TATACAC6600.030.0512683
CAGAGTA55400.029.06451410
CGCAGAG55600.028.8369968
Produced by FastQC (version 0.11.5)