FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369832
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38450.2806913548522738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.038.2961581
GTATCAA39350.037.5384371
TATCAAC43900.033.773922
ATCAACG44000.033.4446833
TCAACGC44150.033.1824464
CAACGCA45250.032.4536365
AACGCAG47400.031.05256
ACGCAGA50050.029.3390567
AGAGTAC50750.028.86709211
CGAATTA2150.028.59637515
CGCAGAG52700.027.9275328
CGTATTC500.00808024327.35441
GCAGAGT53900.027.241429
CAGAGTA53700.027.215710
GTGGTAT7300.026.698641
GAGTACG42500.026.5178612
AGTACGG42650.026.34452213
GTACGGG47150.023.75776714
TACGGGG18150.023.33576215
TGGTATC8600.023.0401762
Produced by FastQC (version 0.11.5)