FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796996
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGG28160.3533267419158942No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTAAATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAAGT24650.30928637032055367No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATTTAGGCTGTCATCCCATGATGCACAGTAATAAT19990.2508168171483922No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCTGGGACCC19700.2471781539681504No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCT16240.20376513809354127No Hit
GTGCTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC15360.19272367740866955No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14620.18343881274184565No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14150.17754166896697096No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATTTAGGCTGTCATCCCATGATGCA13750.1725228232011202No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13360.16762944857941572No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13280.16662567942624557No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13100.16436719883161272No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCAC12960.16261060281356493No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12920.16210871823697987No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12600.15809364162429923No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTC12460.15633704560625147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12110.15194555556113204No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11070.13889655656992003No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10930.13713996055187228No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10900.13676354711943348No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10800.13550883567797078No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10550.13237205707431404No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10350.12986263419138866No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG10340.1297371630472424No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTAA10190.12785509588504837No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10080.1264749132994394No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG10040.12597302872285432No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9720.1219579521101737No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9630.12082871181285729No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGT9470.11882117350651697No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9200.11543345261456771No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGACGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGCTTA9130.11455515460554382No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT8990.11279855858749605No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8950.11229667401091097No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8830.11079102028115574No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8600.10790518396579155No Hit
GCCTAAATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT8550.10727782824506021No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8460.10614858794774377No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8420.10564670337115871No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8250.10351369392067211No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8240.10338822277652586No Hit
CCCAGGGACGGCCCCCAAACTCCTCGTCTATAATAATTATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG8130.1020080401909169No Hit
CTCTTGTTCTGGAAGCAGTTCCAATATCGGAAGGAATGATGTAAGCTGGTACCAGCATCTCCCAGGGACGGCCCC8050.10100427103774674No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC8010.10050238646116165No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8000.1003769153170154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG200.007537202451.4363526
GGTATCA9450.045.7125931
GTATCAA22550.041.3541871
GTGGTAT2350.039.390641
ATCAACG25650.036.4784283
TATCAAC25850.036.3379062
TCAACGC26050.036.049874
CAACGCA26250.035.6468735
TGGTATC2550.034.95452
AACGCAG27200.034.9015276
ATAAACG601.4038984E-534.273693
ACGCAGA27950.033.9649937
AGAGTAC28250.033.2382711
CGCAGAG28600.033.190988
CAGAGTA28550.032.88900810
TCGTATG751.6345439E-632.28455740
GCAGAGT29300.032.16419
GCTTAGT553.3780228E-431.1676771
TCTCGTA802.7460246E-630.20746638
TATACAC1251.3460522E-1030.1608473
Produced by FastQC (version 0.11.5)