FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796996
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGG27090.3399013294922434No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTAAATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAAGT23660.29686472705007305No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATTTAGGCTGTCATCCCATGATGCACAGTAATAAT19790.24830739426546683No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCTGGGACCC19220.24115553904912948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15930.1998755326250069No Hit
GTGCTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC15830.1986208211835442No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15030.18858312965184268No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCT14330.17980014956160384No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC13540.16988792917404855No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13120.16461814111990525No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12820.16085400679551717No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12450.1562115744621052No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATTTAGGCTGTCATCCCATGATGCA12440.1560861033179589No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11850.14868330581332903No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11780.14780500780430517No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11300.14178239288528424No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCAC10950.1373909028401648No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10880.13651260483114092No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTC10700.1342541242365081No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG10660.133752239659923No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10220.12823150931748717No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10080.1264749132994394No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10030.12584755757870805No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTAA10030.12584755757870805No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC9570.12007588494797967No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9540.11969947151554085No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9530.11957400037139458No Hit
CTCTTGTTCTGGAAGCAGTTCCAATATCGGAAGGAATGATGTAAGCTGGTACCAGCATCTCCCAGGGACGGCCCC9420.11819381778578562No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9410.11806834664163937No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9390.11781740435334682No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9250.11606080833529905No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9120.11442968346139755No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8900.11166931829017963No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG8880.11141837600188709No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8850.11104196256944829No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8790.11028913570457065No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGACGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGCTTA8790.11028913570457065No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGT8730.10953630883969304No Hit
GCCTAAATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT8600.10790518396579155No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8060.101129742181893No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8040.10087879989360048No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8020.10062785760530793No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8010.10050238646116165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA200.006697735752.995460
GTTCGCG200.00672524752.94050259
GGTATCA7450.045.052761
GTATCAA20950.043.976421
TCGGCGA350.001185911240.46661462
TATCAAC23800.038.530122
ATCAACG23900.038.3592763
TCAACGC24200.037.8837474
CAACGCA24600.037.2700925
AACGCAG24750.036.9059876
AATGGTA3750.034.94945570
ACGCAGA26250.034.92747
GCTTACC400.002690370234.249291
TGGTATC1800.034.217052
CGCAGAG27250.033.7712068
AGAGTAC27200.033.5838411
CAGAGTA27700.033.0990710
GCAGAGT28200.032.6335229
AGTACGG22100.032.0454313
GAGTACG22300.031.60460712
Produced by FastQC (version 0.11.5)