FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810448
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGGCGGTCACAGAACTCATCTTCAGGGAGA35910.4430882672299765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17830.22000177679505653No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17590.2170404517007877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17070.21062424732987187No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17040.21025408169308826No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16850.2079096993267921No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16700.20605887114287405No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15790.19483051349377137No Hit
GTATATTACTGTGCGAGAGCACGCATCCAGGAGGCTTCGATTAGGTTTGA15510.19137563421712436No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14480.1786666140208872No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13730.16941247310129706No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13360.16484709691429927No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13230.1632430458215703No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12770.15756717272422166No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12660.15620989872268176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12170.15016385998854956No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12010.14818964325903697No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11640.14362426707203918No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11530.14226699307049928No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11170.137825005429096No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11100.13696128560993426No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10870.13412334906125994No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10870.13412334906125994No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10400.12832408741831677No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10270.12672003632558784No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10210.1259797050520206No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTGGACACGTCC10160.12536276232404794No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT10160.12536276232404794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9870.12178449450180641No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9840.1214143288650228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9700.11968688922669932No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9630.11882316940753757No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9520.11746589540599767No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9490.11709572976921408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9370.11561506722207965No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9310.11487473594851243No Hit
CCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAAGATGAGTTCT9250.1141344046749452No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9230.11388762758375613No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9210.11364085049256707No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8910.10993919412473101No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8860.10932225139675833No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG8810.10870530866878567No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGACGCAGACAGGGATGATGGAGACTGGGTCAT8780.10833514303200205No Hit
GTATATTACTGTGCGAGAGCACGCATCCAGGAGGCTTCGATTAGGTTTGACTTCTGGGGCCAGGGAGCCCTGGT8640.10660770339367856No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8630.10648431484808403No Hit
GCCTCTGGTGGGTCTTTTAGTGGTTACTTCTGGAGCTGGATCCGCCAGTC8600.10611414921130043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.10574398357451681No Hit
CTGATATGGTGACTCGACTCTTGAGGGACGGGTTGTAATTGGTCCTTCCCCTATGATTGATTTCTCCAATCCACT8410.10376976684500425No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8400.10364637829940972No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGACAACTATTTAAATTGGTATCATCAGAAG8390.10352298975381519No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8390.10352298975381519No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8380.10339960120822064No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8300.10241249284346436No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCACAGAACTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCCA8250.10179555011549167No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8210.10130199593311354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA200.007026413552.3573547
CCTATCC200.00794529550.7549063
GTGGTAT1800.047.001081
CGTATGC1100.046.96973841
TTATACA1200.045.115472
TATACAC1200.045.115473
CTTATAC1300.044.2533261
TCGTATG1250.041.24786440
GTGTATA1000.040.59891513
TATCTCG1200.039.84979636
GCCTATC1200.039.6964933
GTATCAA13550.038.96061
TGTATAA1050.038.66563414
ATCTCGT1250.038.31547537
AGCCTAT1350.037.7686932
CACTGAT13100.037.68776770
ACGAGAC1450.037.34846522
ATCTCCG1450.037.3346410
GTATGCC1300.037.16875542
CTTGAAA1450.037.037457
Produced by FastQC (version 0.11.5)