FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563620
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCACCAATATGGTGGCTCACCGTGGACGTTCGGCCAAGGGA9500.1685532805791136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7560.13413292643979985No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5960.10574500550015967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12850.043.612631
GGTATCA4600.041.856021
TATCAAC15300.036.6256562
CAACGCA15850.035.1378335
TCAACGC15850.035.1378334
ATCAACG15900.035.0273363
AACGCAG16300.034.167776
AGAGTAC17500.032.02412811
ACGCAGA17400.032.010587
CGCGGTA450.004259356831.18123658
TTAACGG450.0044182830.9487150
CGCAGAG18350.030.3533548
CAGAGTA18700.030.15296410
GCAGAGT18950.029.39239
AGTACGG16500.028.1305113
TAACGGC500.0073742827.8688651
GTGTATA1503.6379788E-1127.50538613
GAGTACG17250.026.7081312
TACGGGC2900.026.0803815
CATAATA700.001360646324.55622
Produced by FastQC (version 0.11.5)