FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563620
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCACCAATATGGTGGCTCACCGTGGACGTTCGGCCAAGGGA9510.16873070508498636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8810.15631098967389376No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7410.13147155885170858No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5680.10077711933572264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13650.043.5483171
GGTATCA5200.040.9681431
TATCAAC16000.036.696582
TCAACGC16650.035.8794634
ATCAACG16650.035.4670563
CAACGCA17000.035.1407665
AACGCAG17400.034.530256
GTATTAA601.379105E-534.3603821
ACGGGTA601.3849885E-534.3390316
AGAGTAC18000.033.37331811
ACGCAGA18350.032.9267587
CGCAGAG18700.032.1240468
CAGAGTA19600.030.47112510
GCAGAGT19850.030.4358889
TACGGGT1600.030.0466515
AGTACGG17450.029.315813
GTGGTAT2400.028.633651
TTTGGCG600.00730180627.9243269
GAGTACG18350.027.87796812
TGGTATC2650.025.9139482
Produced by FastQC (version 0.11.5)