FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082353
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24650.041.1182981
GGTATCA10800.038.4938741
CTCGTAT1350.035.85601839
TCAACGC30100.034.009444
ATCAACG30300.033.7849583
TATCAAC30350.033.7292982
CAACGCA31300.032.9250685
AACGCAG33200.031.2477426
ACGCAGA34400.029.8581227
CGCAGAG34950.029.4865428
AGAGTAC35250.029.1381411
CAGAGTA35800.028.78644210
TCGTATG1601.8189894E-1228.1160840
ACGAGTC500.00789888827.4814578
GCAGAGT39000.026.6006439
AGTACGG31250.025.50161413
GAGTACG31750.025.10117312
GTGGTAT4400.024.987761
CGTATGC1851.8189894E-1124.34039741
TACGGGG15100.023.88592315
Produced by FastQC (version 0.11.5)