FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132856
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT49960.44100927214050156No Hit
GTATTTCTGTCAGCAGTATCATAGGTCACCAGTCACTTTTGGCCAGGGGA35470.3131024596241711No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31300.27629283863085863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26790.2364819535757413No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26570.23453995918280876No Hit
GTATCATAGGTCACCAGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24720.21820955178769413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23610.2084113073506253No Hit
CTCCTGCAGGGCCAGTCAGAATATTAACAGCAACTACTTTGCCTGGTATC22310.19693588593784206No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22000.19419943929325528No Hit
CAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20770.18334192518731418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20630.18210611057362983No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20190.17822212178776473No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGGCTGGAGCCTGAAGATT19120.16877696724032004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18510.1633923464235525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18170.16039108236174765No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT18150.1602145374169356No Hit
CTCCAGGCACCCTGCCTTTGTCTCCAGGGGAAACTGCCACCCTCTCCTGC17230.1520934699555813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16920.1493570233109945No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16410.14485512721828722No Hit
GTCTCCAGGCACCCTGCCTTTGTCTCCAGGGGAAACTGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAACAG16390.1446785822734752No Hit
CCATAGATGAGGAGCCTGGGGGCCTGGCCACGTTTTTGCTGATACCAGGCAAAGTAGTTGCTGTTAATATTCTGA16270.14361931260460287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16080.14194213562888838No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16060.14176559068407635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16020.14141250079445225No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15290.1349686103088124No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15150.13373279569512808No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15120.13346797827791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14920.1317025288297895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14830.13090807657813527No Hit
CCTATGATACTGCTGACAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGT14650.12931917207482682No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14290.12614136306820992No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14120.12464073103730748No Hit
GACTTCACTCTCACCATCAGCGGGCTGGAGCCTGAAGATTTTGCACTGTATTTCTGTCAGCAGTATCATAGGTCA13300.11740238830001341No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13180.11634311863114111No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12910.11395976187617844No Hit
GGCCAAAAGTGACTGGTGACCTATGATACTGCTGACAGAAATACAGTGCA12860.11351839951414833No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12630.11148813264880973No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12320.10875168600422294No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA12210.10778068880775667No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12170.10742759891813258No Hit
CTGCAGGAGAGGGTGGCAGTTTCCCCTGGAGACAAAGGCAGGGTGCCTGG12110.10689796408369642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12010.10601523935963618No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11930.10530905958038797No Hit
GAATATTAACAGCAACTACTTTGCCTGGTATCAGCAAAAACGTGGCCAGG11850.10460287980113978No Hit
CTATGATACTGCTGACAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGTCTGTC11650.10283743035301929No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11520.10168988821174095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGC200.00855507149.8154525
CTTATAC7950.047.5319021
TTATACA7950.047.1149562
TATACAC7950.047.1066363
CGCTAAT10300.036.82885432
CGTATGC10450.036.63907641
TCGTATG10400.036.35993640
ATCTCGT10350.035.54900437
ATGCCGT10950.035.35756744
GCCGTCT10850.035.30953646
TACACAT10950.035.1086925
ACTCGCT10650.035.08212729
TCGCTAA10700.035.05839531
GCTAATC10400.034.926433
CCGTCTT11300.034.64225447
CTTGAAA11700.034.6116457
AAAGGGG11350.034.33096768
ACGAGAC10750.034.24460222
AATCTCG10700.033.94917336
CTAATCT10700.033.74014734
Produced by FastQC (version 0.11.5)