FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132856
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97370.8595090638174667No Hit
GTATTTCTGTCAGCAGTATCATAGGTCACCAGTCACTTTTGGCCAGGGGA33780.2981844117875529No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30910.27285021220702366No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25720.22703679902829665No Hit
GTATCATAGGTCACCAGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24160.21326629333295669No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24050.21229529613649042No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22290.19675934099303No Hit
CTCCTGCAGGGCCAGTCAGAATATTAACAGCAACTACTTTGCCTGGTATC21500.18978581567295402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20790.18351847013212624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.18281229035287805No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.18175302068400573No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGGCTGGAGCCTGAAGATT19770.17451467794671166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18790.16586397565092123No Hit
CAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18550.16374543631317662No Hit
CTCCAGGCACCCTGCCTTTGTCTCCAGGGGAAACTGCCACCCTCTCCTGC17750.1566836385206946No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17050.15050456545227284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16850.14873911600415235No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT16650.14697366655603183No Hit
GTCTCCAGGCACCCTGCCTTTGTCTCCAGGGGAAACTGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAACAG16070.14185386315648238No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15540.13717542211896305No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15130.13355625075031602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15110.13337970580550396No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15040.1327617984986618No Hit
CCTATGATACTGCTGACAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGT15000.1324087086090377No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14860.1311728939953533No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14850.1310846215229473No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14300.12622963554061595No Hit
CCATAGATGAGGAGCCTGGGGGCCTGGCCACGTTTTTGCTGATACCAGGCAAAGTAGTTGCTGTTAATATTCTGA14180.12517036587174363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13600.12005056247219416No Hit
GAATATTAACAGCAACTACTTTGCCTGGTATCAGCAAAAACGTGGCCAGG13350.11784375066204353No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13310.11749066077241944No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13250.11696102593798328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13190.11643139110354714No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13030.11501903154505075No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12940.11422457929339651No Hit
GACTTCACTCTCACCATCAGCGGGCTGGAGCCTGAAGATTTTGCACTGTATTTCTGTCAGCAGTATCATAGGTCA12840.11334185456933626No Hit
CTGCAGGAGAGGGTGGCAGTTTCCCCTGGAGACAAAGGCAGGGTGCCTGG12820.1131653096245242No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12720.11228258490046396No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12600.11122331523159167No Hit
GGCCAAAAGTGACTGGTGACCTATGATACTGCTGACAGAAATACAGTGCA12460.1099875006179073No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA12190.10760414386294463No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11750.10372015507707953No Hit
CTATGATACTGCTGACAGAAATACAGTGCAAAATCTTCAGGCTCCAGCCCGCTGATGGTGAGAGTGAAGTCTGTC11520.10168988821174095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37800.036.2128371
TATCAAC41550.032.8371772
ATCAACG43900.031.2178363
TCAACGC43600.031.1993544
CAACGCA43900.031.061545
AACGCAG44100.030.995726
ACGCAGA45650.030.014477
AGAGTAC46050.029.6100211
CAATCGT500.005897368329.17622255
GCAGAGT48400.028.376239
CGCAGAG49050.028.0001938
ACGAATA606.789908E-427.66521324
CATTACG500.00933088526.55860324
AGTACGG45950.025.85711313
GAGTACG46550.025.59492912
CAGAGTA55250.024.67730310
TGTTAGA13900.023.61474670
GTACGGG50550.023.43867114
TACGGGA17500.023.27282515
ACGGGGA8950.022.94078316
Produced by FastQC (version 0.11.5)