FastQCFastQC Report
Wed 8 May 2024
EGAF00005761112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244261
Total Bases17.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT21890.8961725367537183No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT5500.22516897908384884RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT4320.17685999811676853RNA PCR Primer, Index 29 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)